Introduction

Oral and dental diseases present a major threat to the well-being of our pets, with dogs and cats most commonly suffering from periodontal conditions. Proactive planning for professional dental cleaning and regular at-home oral hygiene will greatly help prevent the establishment and progression of periodontal disease. However, providing good homecare can be challenging, and the ideal of daily tooth brushing is not always easily achieved. Other options for reducing plaque and calculus accumulation are therefore being developed, but the array of products on offer nowadays can make it difficult for the clinician or animal owner to recommend or choose one that is truly effective. The Veterinary Oral Health Council (VOHC) [1] was established to provide a system that delivers an independent review of dental products that effectively reduce plaque and calculus accumulation in dogs and cats, and its Seal of Acceptance can help guide selection of appropriate health care products.

Oral and dental diseases – a welfare issue

A recent study determined that oral and dental pathologies rank in the top three conditions that have a significant welfare impact on dogs, based on their high prevalence, duration and severity [2], a view shared by the World Small Animal Veterinary Association [3]. Among the various oral and dental diseases, periodontal conditions are the most common and will affect most dogs and cats at some point in their lives [4] [5] [6] [7]. Periodontal problems progress with age, some individuals, especially small breeds of dog, can be affected before one year of age [5] [6]. Despite the high prevalence of chronic inflammatory periodontal disease in companion animals, the causal mechanisms are only now being more extensively revealed. As in humans, a multifactorial etiology is suspected, with the oral cavity microbiome being the major trigger for the onset, and host factors and environmental conditions involved in their development and progression [8] [9] [10] [11] [12].

Tooth brushing – the best preventative option

One of the main approaches to the prevention of periodontal disease is to inhibit development of dental plaque; this includes professional dental cleaning (Figure 1) and home oral hygiene. 

Guidelines for appropriate in-clinic diagnostic procedures and treatment are well established [3], whilst the gold standard of home oral hygiene remains daily tooth brushing (Figure 2), which reduces the amount of plaque and calculus [13] [14] and inhibits oral bacterial growth [15]. Daily brushing has also been shown to be superior in efficacy at controlling plaque accumulation in dogs when compared to a daily dental chew or dental diet [16], and the importance of regular homecare in prevention of periodontal disease development has been shown even in very young dogs [5] [6].

However, tooth brushing may not always be realistic, and other practical alternatives that help reduce plaque and calculus accumulation, and at the same time promote the proportion of “healthy” oral bacteria, need to be considered [5] [17]. Moreover, ongoing education between veterinarians, veterinary nurses, and pet owners is needed to increase compliance with home oral hygiene recommendations [18] [19].

VOHC – safe and effective dental products 

However, VOHC is not a regulatory agency, and when it authorizes the use of its Registered Seal on a certain product, this only means that a product has met its standards for effectiveness when used as directed by the manufacturer. VOHC also requires the manufacturer to ensure that any product evaluated is safe (i.e., it does not cause trauma to the oral tissues, or any extra-oral problems such as toxicity, esophageal and/or gastrointestinal obstruction or perforation, and/or nutritional imbalance) and meets all applicable regulatory requirements. Once a VOHC^®; Seal of Acceptance has been issued the manufacturer must ensure that these conditions continue to be met, and that any complaints from consumers are promptly reported to the VOHC.

The Seal of Acceptance may be extended to other products in a product line if there are only minor differences between them (e.g., flavor) that do not change the dental effectiveness. However, if major differences exist, (for example, if they manufacture the product in a different shape or with varying ingredients, or if they plan to market the product in alternative sizes for dogs of different body sizes) additional or more complex trials are needed.

Testing products for the VOHC^®; Seal of Acceptance

All products are tested on either dogs or cats depending on their intended use. Trial design and analysis procedures must be carefully described and documented, but the protocol will vary depending on the product type to ensure appropriate comparisons between the test groups. For example, for chewed and ingested mechanically-effective products (i.e., dental diets, chews and treats) two groups of animals are required – a test group and a control group. All trial groups must have the same number of weight- and age-matched animals randomly assigned at the start of the study. There is no stated minimum number of animals in each group, as this can vary between products, but appropriate statistical analyses must be used according to the data distribution. Two trials are required to ensure the results are reproducible, and animals from one trial cannot be included in the second trial, although cross-over studies are permitted where appropriate. This study design offers the advantage that animals act as their own controls and therefore fewer animals per group may be needed, but any possible carry-over effects must be included when it comes to the data analysis. 

Animals in a trial may be either pets (with informed consent from the owner) or from an USDA-recognized (or similar) facility (with approval of the institute’s Animal Care and Use Committee). All animals must be clinically evaluated to ensure inclusion criteria are met – i.e., in good general health and without severe periodontal and/or dental disease (Figure 4), especially on the teeth to be used for evaluation of plaque and calculus accumulation (referred to as “VOHC teeth”). In addition, certain standard criteria are required to ensure a valid study (Table 1).

On day zero of the trial the teeth are scaled (Figure 1) and polished using a standard state-of-the-art technique, and a disclosing solution applied at the end of the procedure to ensure complete removal of plaque and calculus (i.e., a “clean tooth model” (Figure 5 and 6)). At the end of the trial period the selected teeth are scored again for plaque and calculus accumulation and any associated gingivitis, along with any other non-gingival inflammation or trauma that has occurred during the trial. Whilst a Gingivitis Index is provided to indicate product safety, VOHC does not support any product claim for reducing gingivitis.

Test result evaluation

Acknowledgements
Ana Nemec has been a VOHC Council member since 2019.

Conclusion